cytochrome C from bison heart
Cytochrome C
CAS: 9007-43-6
Molecular Formula: C42H52FeN8O6S2
cytochrome C from bison heart - Names and Identifiers
cytochrome C from bison heart - Physico-chemical Properties
| Molecular Formula | C42H52FeN8O6S2 |
| Molar Mass | 884.88728 |
| Melting Point | 300 °C |
| Water Solubility | 100 g/L (20 ºC) |
| Solubility | Water |
| Appearance | Deep red to reddish brown powder |
| Merck | 13,2820 |
| Storage Condition | -20°C |
| Physical and Chemical Properties | The reduction type is dispersed needle-like crystals, and the oxidation type is petal-like crystals. Both are easily soluble in water and acidic solutions. The former aqueous solution is pink and the latter is dark red. The heat is relatively stable. The former is more stable than the latter, with a molecular weight of about 11000-13000. |
| Use | Used as a cell respiration activating agent |
cytochrome C from bison heart - Risk and Safety
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S37/39 - Wear suitable gloves and eye/face protection S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| RTECS | HA5365000 |
| FLUKA BRAND F CODES | 1-8-10 |
| TSCA | Yes |
| HS Code | 35040000 |
cytochrome C from bison heart - Reference
| Reference Show more | 1. Zhang Yifan, Wang Qiang, Wang Wei, etc. Study on the stability of antioxidant and hypoglycemic activity of Myrica rubra proteolytic peptides [J]. Science and Technology of Food Industry, 2015, 36(014):86-91. 2. Zeng Dujuan, Wu Xiaolin, Wu nengjian, etc. Resistance of Aedes albopictus larvae in different habitats in Pingshan district of Shenzhen [J]. Modern Preventive Medicine, 2019, Volume 46, Issue 14, pages 2653-2657, ISTIC PKU CA. 3. Liu Xiaona, Wang Dequan, Zeng Dujuan, etc. Preliminary Study on resistance and mechanism of Aedes albopictus in Pingshan district of Shenzhen [J]. Modern Preventive Medicine, 2018, 45(21):155-158. 4. LV Juan, Zhou Gang, Li Zhenlian, Du Yan, Zhu Yeqing. Determination of molecular weight distribution of polypeptides in peptone by gel filtration chromatography [J]. Journal of Food Safety and quality testing, 2020, 11(15):5131-5136. 5. Kuang Cong, Jia Junqiang, Wu qionying, et al. Effect of ultrasonic-ionic liquid treatment on enzymatic hydrolysis kinetics and antioxidant activity of whey protein [J]. Food Science, 2017, 29 (23):32-38. 6. Jin Jian, Ma Haile, Wenjuan Qu, et al. Effect of ultrasonic pretreatment on enzymatic hydrolysis of corn protein [J]. Chinese Journal of cereal and oil, 2015, 30(11):58-64. 7. Zhang Miao, Jiang, Yu, Tang, et al. Effects of cold shock combined with Betaine treatment on chilling injury and energy metabolism of summer squash [J]. Food science 2020 v.41;No.620(07):193-199. 8. Liu Xie, Guo Yunlong, Xu Qingxuan, etc. Study on the analytical properties of melon seed shell as a new type of wood electrospray material [J]. Analytical Chemistry, 2019(9). 9. Zhang Zhengmin, Yang Yilin, Li Meilin, et al. Effects of 2 4-epibrassinolide treatment on soft rot and energy metabolism of peach fruit [J]. Food science 2019 v.40;No.594(05):215-221. 10. Zhang He. Research and construction of enzyme biofuel cell and bio-photoelectrochemical system [D]. University of Science and Technology of China, 2020. 11. Wang, C., li, M., wang, P. et al. An electrochemiluminescence biosensor based on boron nitride quantum dots as novel coreactant for quantitative determination of concanavalin A. Microchim Acta 187, 409 (2020). https://doi.org/10.1007/s00604-020-04385-6 12. Huang, Shan, Ming Lu, and Lei Wang. "Cytochrome c-multiwalled carbon nanotube and cobalt metal organic framework/gold nanoparticle immobilized electrochemical biosensor for nitrite detection ." RSC Advances 11.1 (2021): 501-509.10.1039/D0RA09551F 13. Liu, Xiuhui, et al. "Enzymes immobilized on amine-terminated ionic liquid-functionalized carbon nanotube for hydrogen peroxide determination." Talanta 105 (2013): 63-68.https://doi.org/10.1016/j.talanta.2012.11.059 14. Xu, Wei, et al. "Creating magnetic ionic liquid-molecularly imprinted polymers for selective extraction of lysozyme." RSC advances 8.39 (2018): 21850-21856.10.1039/C8RA03818J 15. Wang, C., Li, M., Wang, P. et al. An electrochemiluminescence biosensor based on boron nitride quantum dots as novel coreactant for quantitative determination of concanavalin A. Microchim Acta 187, 409 (2020). https://doi.org/10.1007/s00604-020-04385-6 16. [IF=5.833] Zheng Jiushang et al."Nanostructured MXene-based biomimetic enzymes for amperometric detection of superoxide anions from HepG2 cells."Microchim Acta. 2019 Feb;186(2):1-9 17. [IF=3.205] Dandan Lan et al."Preparation of a hydroxyethyl-based monolithic column and its application in the isolation of intact proteins from complex bio-samples."J Chromatogr B. 2019 Jan;1104:89 18. [IF=5.833] Wang Caixia et al."An electrochemiluminescence biosensor based on boron nitride quantum dots as novel coreactant for quantitative determination of concanavalin A."Microchim Acta. 2020 Jul;187(7):1-9 19. [IF=4.616] Yufeng Liu et al."In vitro measurement of superoxide dismutase-like nanozyme activity: a comparative study."Analyst. 2021 Mar;146(6):1872-1879 20. [IF=5.396] Hongdong Song et al."Digestion characteristics of quinoa barley and mungbean proteins and the effects of their simulated gastrointestinal digests on CCK secretion in enteroendocrine STC-1 cells."Food & Function. 2022 May ;: |
cytochrome C from bison heart - Nature
Open Data Verified Data
- This line was extracted from pig or bovine heart. The aqueous solution is a clear solution of deep red color. Cytochromes are binding proteins of iron-containing porphyrins in a ratio of 1:1. Each molecule contains one iron atom, so the iron content is 43% of the molecular weight. Protein to lysine-based Basic amino acid content is more, it is alkaline. It is stable to dryness, heat and acid. The molecular weight of cytochrome C in porcine heart was 12200, and the molecular weight of cytochrome C in yeast was about 13000. The pH of isoelectric point was 10. 2~10.8.
- cytochrome C is a very important electron transport agent for biological oxidation. Usually exogenous cytochrome C can not enter healthy cells, but in hypoxia, the permeability of cell membrane increases, cytochrome C may enter cells and mitochondria, enhance cell oxidation, increased oxygen utilization. For the first-aid and adjuvant treatment of various tissue hypoxia.
Last Update：2024-01-02 23:10:35
cytochrome C from bison heart - Preparation Method
Open Data Verified Data
- extraction method using pig heart as raw material. Wash fresh or frozen pig heart, remove blood clots, fat and tendons, cut, minced with a meat grinder. Then, 1.5 times of water was added, and the pH value was adjusted to about 4 with 1mol/L sulfuric acid. The mixture was stirred at room temperature, extracted for 2H, and press-filtered. The filtrate was neutralized with 1mol/L aqueous ammonia to pH 6.2, centrifuged, and a clear solution was separated. The residue was repeatedly extracted once with an equal amount of water, and the extracts were combined twice. The extract was adjusted to pH 7.5 with 2mol/L ammonia water, and the mixed proteins were precipitated statically, centrifuged, and the supernatant was drawn. Then, lOg zeolite was added per liter of the extract solution, fully adsorbed for 40min under constant stirring, allowed to stand, and the supernatant was discarded. The zeolite adsorbed with cytochrome C was washed 3 times with distilled water, washed 4 times with 0.2% sodium chloride, and then washed with distilled water until the washing solution was clear. The zeolite was filtered, drained, loaded into a column, and eluted with 25% ammonium sulfate solution to collect the eluate. Solid ammonium sulfate was added to the eluate to a concentration of 45%, and the heterologous protein was completely precipitated and filtered. A solution of trichloroacetic acid (20%) was slowly added to the filtrate, followed by stirring. After the cytochrome C precipitate was completely precipitated, the precipitate was collected by centrifugation. The precipitate was dissolved in distilled water and dialyzed with water to sulfate-free. The post-dialysis solution was adsorbed through a column of treated Amberlite IRC-50( NH +4) resin, washed with water until clear, and then treated with disodium hydrogen phosphate (0. 6mol/L) and sodium chloride (0.4mol/L) mixture elution, elution rate is slow, the general flow rate of 2 ml/min. The eluate is dialyzed with distilled water until no chloride ion is present, and cytochrome C fine solution can be obtained. The yield was 100 mg/kg and the content was more than 20mg/mL.
- extraction method using fresh yeast as raw material.
- injection I preparation method.
Last Update：2022-01-01 11:11:10
cytochrome C from bison heart - Standard
Authoritative Data Verified Data
aqueous solution of cytochrome C extracted from pig or bovine heart in this line. Cytochrome C should not be less than 15mg per lml.
Last Update：2024-01-02 23:10:35
cytochrome C from bison heart - Trait
Authoritative Data Verified Data
This product is a clear liquid of deep red color.
Last Update：2022-01-01 13:38:18
cytochrome C from bison heart - Use
Open Data Verified Data
activator of cellular respiration. For tissue hypoxia first aid and adjuvant medication. It is suitable for treating a series of symptoms caused by cerebral hypoxia, myocardial hypoxia and other tissue hypoxia. This product can promote the regeneration of damaged liver cells, bone marrow hematopoietic function repair, significantly reduce the leukopenia caused by radiotherapy.
Last Update：2022-01-01 11:11:10
cytochrome C from bison heart - Differential diagnosis
Authoritative Data Verified Data
- 1ml of the sample solution containing iron was taken, and a 20% trichloroacetic acid solution was added dropwise, I .e., a brown or brownish-red curd-like precipitate was formed, and the red color of the solution disappeared. The precipitate can be dissolved in water, and the solution is brownish red.
- take 1ml of the test solution under the item of iron content, put it in a 50ml measuring flask, and use phosphate buffer solution (take 1.38g of sodium dihydrogen phosphate and 31.2g of disodium hydrogen phosphate, add appropriate amount of water to dissolve it into 1000ml, adjust pH to 7.3) dilute to the scale, add about 15mg sodium dithionite, shake, UV-Vis spectrophotometry (General 0401) determination, there is a maximum absorption at wavelengths of 520nm and 550nm, and a minimum absorption at a wavelength of 535nm.
Last Update：2022-01-01 13:38:19
cytochrome C from bison heart - Safety
Open Data Verified Data
- low toxicity, mice intravenous injection, LD50 3~6 g/kg. There are local cramps, rashes, Fever, thirst, temporary Shock and allergies. Allergic reactions should be discontinued immediately. Allergy test should be carried out before use, positive should not be used. Preparation:(1) cytochrome C injection;(2) cytochrome C for injection.
- sealed and stored at below 4 °c.
Last Update：2022-01-01 11:11:11
cytochrome C from bison heart - Exam
Authoritative Data Verified Data
preparation of iron standard solution
50g of ammonium ferric sulfate was taken, and a mixed solution of 300ml of water and 6mL of sulfuric acid was added to dissolve the solution, and then an appropriate amount of water was added to make 1000ml, followed by shaking. Take 25ml accurately, put in the iodine bottle, add hydrochloric acid 5ml, mix, add potassium iodide solution 12ml, plug, stand for 10 minutes, with sodium thiosulfate titration solution (0.1 mol/L) titration, when the end point was reached, 1ml of starch indicator solution was added, and the titration was continued until the blue color disappeared. Each 1ml of sodium thiosulfate titration solution (0.1 mol/L) corresponds to 5.585mg of Fe. The Fe content (mg) per 1ml was calculated from the amount (ml) consumed in the sodium thiosulfate titration solution (0.1 mol/L). An appropriate amount was accurately measured, and a sulfuric acid diluent (2ml of dilute sulfuric acid and 500ml of water) was added to prepare Fe containing 23ug per 1 ml.
preparation of test solution
take an appropriate amount of this product (about equivalent to cytochrome C lOOmg), put it in a 10ml measuring flask, and dilute it with water to the scale.
assay
take 5ml of the test solution, place it in a crucible that has been burned to constant weight, steam dry, dry it to constant weight at 105°C, and weigh it accurately, after slowly burning to complete charring, continue burning at 500-600°C to make complete ash and constant weight, and precisely weigh W2. Another precision sample solution lml, put in 25ml measuring flask, add 30% hydrogen peroxide solution 0.5ml and dilute sulfuric acid ml, place in water bath, heat for 30 minutes, take out, let cool, add 2ml of human bipyridine solution in a cold water bath, slowly add 5ml of sodium sulfite solution, shake with addition, place in a water bath at 60-70°C, heat for 30 minutes, remove, and let cool to room temperature, dilute to scale with water, shake well, measure absorbance A1 at the wavelength of 522nm by UV-Vis spectrophotometry (General rule 0401), and take 2ml of standard iron solution with precision, set 25ml measuring flask, according to the above method, from the "add 30% hydrogen peroxide solution 0.7", operate according to law, measure absorbance A2, iron content should be 0.40% ~ 0.46%.
bacterial endotoxin
take this product, check according to law (General 1143), each 1 mg of cytochrome C containing endotoxin should be less than 5.0EU. Allergic reaction take an appropriate amount of this product, diluted with water for injection into a solution containing 1147 mg of cytochrome C per 1 ml, as a test solution, according to the law (general rule), should comply with the provisions.
vitality
not less than 1206 as determined by cytochrome C assay (General 95.0%).
Last Update：2022-01-01 13:38:20
cytochrome C from bison heart - Content determination
Authoritative Data Verified Data
take 1 ml of the test solution under the item of iron content, put it in a 50ml measuring flask, dilute it to the scale with the phosphate buffer under Item (2), and add about 15mg of sodium hydrosulfite, shake, according to UV-visible spectrophotometry (General rule 0401), at a wavelength of about 550nm, with an interval of 0.5nm to find the maximum absorption wavelength, determine the absorbance, it is obtained by calculating the absorption coefficient of cytochrome C as 23.0.
Last Update：2022-01-01 13:38:20
cytochrome C from bison heart - Category
Authoritative Data Verified Data
cell metabolism improving drugs.
Last Update：2022-01-01 13:38:20
cytochrome C from bison heart - Storage
Authoritative Data Verified Data
sealed and stored at 4°C or lower.
Last Update：2022-01-01 13:38:21
cytochrome C from bison heart - Legal requirements
Authoritative Data Verified Data
This product should be extracted from Quarantine qualified pigs or cattle heart, the species of animals used should be clear, the production process should comply with the current version of the "good manufacturing practice" requirements.
Last Update：2022-01-01 13:38:21
cytochrome C from bison heart - Cytochrome C injection
Authoritative Data Verified Data
- This product is a sterile aqueous solution of cytochrome c. The content of cytochrome C should be 90.0% to 110.0% of the labeled amount.
- This product plus equal amount of diglycyl peptide as stabilizer, add sodium bisulfite or sodium sulfite as antioxidant.
trait
This product is a clear liquid of orange-red color.
identification
- 1ml of this product was taken, and the same reaction was shown in the identification (1) Test under the item of cytochrome C solution.
- the solution after the measurement under the content measurement item was taken, and the same result was shown in the test of the identification item (2) under the cytochrome C solution item.
examination
- the pH value should be 6.0 to 7.5 (General 0631).
- bacterial endotoxin and allergic reaction should be checked according to the method under cytochrome C solution, and should be in accordance with the regulations.
- The activity shall not be less than 1206 as determined by cytochrome C assay (General 90.0%).
- others should comply with the relevant provisions under injection (General 0102).
Content determination
take 1ml of this product with precision, according to the method under the item of cytochrome C solution, from "put in 50ml measuring flask", and measure it according to law.
category
Same as cytochrome C solution.
specification
2ml:15mg
storage
sealed and kept in a cool dark place.
Last Update：2022-01-01 13:38:22
cytochrome C from bison heart - Cytochrome C for injection
Authoritative Data Verified Data
This product is a sterile product obtained by freeze-drying cytochrome C with suitable excipients and antioxidants. The content of cytochrome C should be 90.0% to 115.0% of the labeled amount.
trait
This product is a pink freeze-dried cake.
identification
- take 1 piece of this product, add 5ml of water to dissolve, take 1ml, according to the identification (1) Test under the item of cytochrome C solution, show the same reaction.
- the solution after the measurement under the content measurement item was taken, and the same result was shown in the test of the identification item (2) under the cytochrome C solution item.
examination
- the pH value of this product is taken, dissolved and diluted with water for injection to prepare a solution containing 3mg per lml, which is determined according to law (General rule 0631). The pH value should be 6.0-7.5.
- sterile take this product, after dissolving with appropriate solvent, the membrane filtration method, according to the law inspection (General 1101), should comply with the provisions.
- bacterial endotoxin and allergic reaction should be checked according to the method under cytochrome C solution, and should be in accordance with the regulations.
- The activity shall not be less than 1206 as determined by cytochrome C assay (General rule 90.0%).
- others should comply with the relevant provisions under injection (General 0102).
Content determination
take 5 pieces of this product, add cytochrome C solution to identify (2) under the appropriate amount of phosphate buffer solution to dissolve, and transfer the whole amount to the same 100ml measuring flask, dilute to the scale with the above phosphate buffer and shake. 2ml was accurately measured, placed in a 10ml measuring flask, diluted to the scale with the above phosphate buffer solution, and measured according to the law according to the method under cytochrome C solution, starting from "adding about 15mg of sodium dithionite.
category
Same as cytochrome C solution.
specification
15mg
storage
sealed and kept in a cool dark place.
Last Update：2022-01-01 13:38:23
cytochrome C from bison heart - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Use | cell respiration activating drug. It has a rapid enzymatic effect on the oxidation and reduction processes of cells in tissues. For first aid or adjuvant therapy due to various causes of tissue hypoxia. It also has a certain therapeutic effect on the reduction of white blood cells caused by anticancer drugs, circulatory disorders of the limbs, liver diseases and nephritis. succinic acid-CoA derived from the tricarboxylic acid cycle has only 104 amino acids in its peptide chain and is abundant in the body without exogenous supplement; cytochrome C is a very important electron transport body for biological oxidation. It is arranged in the respiratory chain with other oxidases on the mitochondria and participates in the process of cell respiration. The cell membrane permeability is higher in liver cell inflammation, and cytochrome C can enter human cells. Can treat liver failure, increase cell oxidation, improve oxygen utilization. Is an iron-containing binding protein with antigen. used as a cell respiration activator |
| production method | Method 1: myocardium as raw material. Method 2: Using yeast as raw material. |
Last Update：2024-04-09 21:01:54
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Featured ProductsMultiple SpecificationsSpot supply
Product Name: Cytochrome C Visit Supplier Webpage Request for quotationCAS: 9007-43-6
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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